When cutaneous injuries occur, skin homeostasis and integrity are damaged, leading to dire consequences such as acute, chronic, or infected wounds. The content of this article was expressly written by the author(s) listed. Safety and Wound Outcomes Following Genetically Corrected Autologous Epidermal Grafts in Patients With Recessive Dystrophic Epidermolysis Bullosa. -, Aiuti A, Biasco L, Scaramuzza S, Ferrua F, Cicalese MP, Baricordi C, Dionisio F, Calabria A, Giannelli S, Castiello MC, et al. Please enable it to take advantage of the complete set of features! Note the presence of KI67-positive keratinocytes (red) in the basal and suprabasal layers in the corrected epidermis at 385 days post-transplantation, indicating that transplanted cells had self-renewed for more than a year. Immunodeficient SCID mice were transplanted with untransduced cultured RDEB keratinocytes (left) or the COLVII-secreting holoclone (clone 6) (right). A Single Epidermal Stem Cell Strategy for Safe Ex Vivo Gene Therapy - PubMed There is a widespread agreement from patient and professional organisations alike that the safety of stem cell therapeutics is of paramount importance… NLM Extensive growth potential of recessive dystrophic epidermolysis bullosa (RDEB) epidermal keratinocytes. "It's very similar to what we've been using for full-thickness burns: Take a biopsy and isolate cells. Tissue Engineered Skin and Wound Healing: Current Strategies and Future Directions. HHS Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice. Thanks to a discovery made in 1970 by Professor Howard Green in the USA, epidermal stem cells can be taken from a patient, multiplied and used to grow sheets of epidermis … 2013;341:1233151. Although they serve a number of important functions, their primary role is to protect from a variety of harmful factors … Narrow-band UVB treatment: Four weeks after transplantation of epidermal cells, the grafted and control patch will be treated by Narrow-band UVB. The latter corresponded to proviruses. Lower panel: the phosphorylation state of the PRB restriction point was maintained in clone 6 and untransduced recessive dystrophic epidermolysis bullosa (RDEB) cells, whereas PRB was heavily phosphorylated in transformed cells (SCC-13). Base Editor Correction of COL7A1 in Recessive Dystrophic Epidermolysis Bullosa Patient-Derived Fibroblasts and iPSCs. Wang C, Wang M, Xu T, Zhang X, Lin C, Gao W, Xu H, Lei B, Mao C. Theranostics. COVID-19 is an emerging, rapidly evolving situation. The epidermal cells perform a barrier function in human body, protecting against invasion of bacteria and foreign particles and regulating … Significance: Skin serves as a protective barrier for mammals. Identification of sequence abnormalities in the two alleles of the DARS-targeted gene in clone 6 by NGS. Clipboard, Search History, and several other advanced features are temporarily unavailable. The grafts grew, thanks to included epidermal stem cells. Ojeh N, Pastar I, Tomic-Canic M, Stojadinovic O. Int J Mol Sci. Western blotting revealed that only clone 6 secreted COLVII in the culture supernatant, while clone 54 and surprisingly clone 22 did not (see A). Epidermal stratification in mouse model.…, Epidermal stratification in mouse model. 2021 Jan 7;12(1):42. doi: 10.1186/s13287-020-02106-7. The progeny of the selected stem cells also had a diploid karyotype, was not tumorigenic and did not disseminate after long-term transplantation onto immunodeficient mice. Dual sgRNA CRISPR/Cas9-Mediated gene Editing and iPSCs strategy is a powerful and efficient means of by-passing the heterogeneity of transduced! 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